GAG (glycosaminoglycan) analogue realizing cell membrane modification, synthetic method of GAG analogue and application method of GAG analogue in in-vitro induced stem cell directional differentiation

• Main Authors: CHEN GAOJIAN, CHEN HONG, YUAN LIN, LIU QI, CAO LIMIN, LYU ZHONGLIN
• Format: Patent
• Language: Chinese; English
• Published: 13.07.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The invention discloses a GAG (glycosaminoglycan) analogue realizing cell membrane modification, a synthetic method of the GAG analogue and an application method of the GAG analogue in in-vitro induced stem cell directional differentiation. The GAG analogue can be inserted into the surface of a cell membrane and can prompt a stem cell to be directionally and efficiently differentiated into a mature neuronal cell in a shorter cycle. The phospholipid anchored GAG analogue is prepared firstly through RAFT (reversible addition-fragmentation chain transfer) polymerization, and then phospholipidation of the GAG analogue is realized through maleimidized lipid molecules; the phospholipidized GAG analogue is introduced to the surface of the stem cell through affinity interaction between phospholipid groups and the cell membrane, so that the stem cell can be better prompted to be efficiently differentiated into the mature neuronal cell. A synthetic process is simple, and a powerful means capable of realizing phospholip