Preparation method of PRP (platelet-rich plasma)/MSCs (marrow stromal cells)/oyster shell bone tissue scaffold material

• Main Authors: PENG LEI, CHEN LONG, FENG YONGZENG, CHEN HUI, YANG LEI, XUE ENXING, FANG XIAOQIU, SHI CHENGDI
• Format: Patent
• Language: Chinese; English
• Published: 13.07.2016
• Subjects: CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL

Disclosed is a preparation method of a PRP (platelet-rich plasma)/MSCs (marrow stromal cells)/oyster shell bone tissue scaffold material.A tissue engineering bone is constructed by taking oyster calcium carbonate as a scaffold, MSCs as seed cells and a PRP as an endogenous growth factor.The preparation method is simple and convenient.The tissue engineering bone, constructed by taking oyster shell as a scaffold, the marrow stromal cells as the seed cells and the platelet-rich plasma as the endogenous growth factor, is excellent in bone defect repairing effect and is similar to an autogenous bone graft, thereby being a satisfactory bone graft substitute material.A membrane-scaffold complex formed by wrapping the oyster shell scaffold by a membrane has excellent physical, chemical and mechanical properties, and is excellent in ectopic bone formation capability and biocompatibility, good in adhesive capacity for cells, capable of keeping normal growth, differentiation, proliferation and synthesizing and secreting