Method for determining toxicity of pesticide water dispersible granules by model biological alga

• Main Authors: LUO YUANHUA, ZHANG DEYONG, XI QING, LIU ZHIBANG, CHEN YUAN, LIU JIANYU, CHEN ANG, LUO XIANGWEN, YAN QINGPING, JIANG GUIFANG, LUO JIE, HE ANDI, LIU YONG
• Format: Patent
• Language: Chinese; English
• Published: 06.07.2016
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for determining the toxicity of pesticide water dispersible granules by model biological alga. The method comprises the following steps: preparing the pesticide water dispersible granules into a testing pesticide solution with a gradient concentration; diluting biological algae raw solution with sterile water to prepare an algae diluting solution; mixing the testing pesticide solution with the algae diluting solution; culturing at a sealed condition for 72h; counting up algae cells every 24h, and recording the biological effect of the algae cells and physicochemical parameters of the testing solution; calculating the median effective concentration EC50 on the algae cells of the pesticide water dispersible granules according to biological effect parameters of the algae cells; and judging the toxicity of the pesticide water dispersible granules. The method disclosed by the invention has the advantages of easiness in operation, strong practicability, short period and easiness in