Naked mole rat cortical neuron culture method

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat cortical neurons. The cortical neurons are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by compreh...

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Main Authors YANG WENJING, CUI SHUFANG, CHENG JISHUAI, SUN WEI, TANG QIU, CONG WEI, ZHAO SHANMIN, XIAO BANG
Format Patent
LanguageChinese
English
Published 22.06.2016
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat cortical neurons. The cortical neurons are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat cortical neurons is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat cortical neurons with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat cortical neurons can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basi
Bibliography:Application Number: CN20161164387