Preparing method and detecting method for MRJR1 antibody pairs in honey and kit
The invention discloses a preparing method and a detecting method for MRJR1 antibody pairs in honey and a kit. The amino acid sequence of the specific polypeptide M4 of MRJR1 is IKEALPHVPIFD, the IKEALPHVPIFD is shown in SEQ ID NO:1; the amino acid sequence of the specific polypeptide M5 of the MRJR...
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Main Authors | , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
22.06.2016
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a preparing method and a detecting method for MRJR1 antibody pairs in honey and a kit. The amino acid sequence of the specific polypeptide M4 of MRJR1 is IKEALPHVPIFD, the IKEALPHVPIFD is shown in SEQ ID NO:1; the amino acid sequence of the specific polypeptide M5 of the MRJR1 is SGEYDYKNNYPSDID, and the SGEYDYKNNYPSDID is shown in SEQ ID NO:2; New Zealand white rabbits are immunized. An ELLSA double-antibody sandwich method includes the following steps that a caught antibody SP-1 is coated, sealed and washed, an antigen is added, an enzyme-labeled detecting antibody HRP-SP-2 of an is added, washing is carried out, a color rendering substrate is added, a color rendering stopping solution is added, and the light absorption value of 450 nm-630 nm of a sample is measured through an enzyme-labeling instrument. The antigen is to-be-detected honey or pure honey of a known honey source, and the quality of the to-be-detected honey is judged by comparing the light absorption value of the MRJR1 |
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Bibliography: | Application Number: CN20161146713 |