Preparation method of CSF (Classical Swine Fever) and PR (Pseudorabies) bivalent live vaccine and product of CSF and PR bivalent live vaccine

• Main Authors: NING YIBAO, LIN XUYE, ZHANG GUOLI, WU WENFU, YOU QIYOU, LAI YUEHUI, YANG AOBING, QIU CHENGWEN, ZHANG YUJIN, CEN XIAOQING, REN XIANGYANG
• Format: Patent
• Language: Chinese; English
• Published: 18.05.2016
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention provides a preparation method of a CSF (Classical Swine Fever) and PR (Pseudorabies) bivalent live vaccine and a product of the CSF and PR bivalent live vaccine. The method comprises the following steps of culturing a CSF virus lentogen strain and a PR virus lentogen strain by a cell roller bottle or a bioreactor; harvesting obtained cell culture virus solutions; mixing the two kinds of obtained cell culture virus solutions according to a certain volume percent; adding a stabilizing agent and antibiotics into the mixed solution; and performing freeze-drying to obtain the CSF and PR bivalent live vaccine. The CSF and PR bivalent live vaccine is used for immunization; the workload of the immunization can be reduced; the immunization times can be reduced; the immunologic paralysis due to frequent immunization is avoided; the stress on swinery is correspondingly reduced; and the goal of preventing two kinds of diseases by one vaccine is achieved.