Gray mold resistance identified seedling stage inoculation method for capsicum

• Main Authors: HUANG RONG, HUANG RUIRONG, CHEN XUEJUN, MIAO NANSHENG, HU JIANKUN, FANG RONG
• Format: Patent
• Language: Chinese; English
• Published: 11.05.2016
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED; ENZYMOLOGY; FISHING; FORESTRY; HORTICULTURE; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TRAPPING; VINEGAR; WATERING; WINE

The invention relates to a gray mold seedling-stage inoculation identification method for capsicum and belongs to the technical field of plant protection. The method comprises the steps of culturing botrytis cinerea, collecting botrytis cinerea spores, preparing a 10 /mL botrytis cinerea spore suspension, sowing capsicum seeds in sterilized soil, putting the capsicum seeds into a greenhouse with the temperature of 20-25 DEG C, dropwise adding the spore suspension to seedling center growth points for inoculation after each seedling grows to a four-leaf period, carrying out closed moisture preserving on the greenhouse, and carrying out gray mold investigation 21 days later. The method has the technical characteristics of seedling stage identification, simplicity and convenience in operation, high efficiency, rapidness and accurate and reliable identification results. The method is mainly applied to the fast screening of disease-resistant germplasm from capsicum resource varieties and the disease resistance iden