Genetic marker related to porcine semen quality traits and application

• Main Authors: LI JIALIAN, LI FENGE, NIU BUYUE, MA CHANGPING, SONG HUIBIN, HU PANDI
• Format: Patent
• Language: Chinese; English
• Published: 27.04.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention belongs to the technical field of screening and application of pig genetic markers, and in particular relates to a genetic marker related to porcine semen quality traits and application, the genetic marker is a gene obtained by cloning of a pig RNF4 gene 3 'untranslated region, the single nucleotide polymorphism (SNP) can be used as a genetic marker of the porcine semen quality traits, a nucleotide sequence of the genetic marker is as shown in SEQ ID NO: 3 or SEQ ID NO: 4, the sequence SEQ ID NO: 3 or SEQ ID NO: 4 has an allelic gene mutation at 487bp, and AluI-RFLP polymorphism is caused by the allelic gene mutation. Screening step of the method are as follows: extraction of genomic DNA from pig blood, primer design, PCR amplification, PCR product cloning, sequencing, sequence comparing analysis, single nucleotide polymorphism detection, marking and semen quality trait correlation analysis. The genetic marker can be used in pig marker assisted selection.