Method for fermented production of succinic acid on the basis of electrochemical system for regulating intracellular reducing power regeneration

• Main Authors: ZHANG MIN, MA JIANGFENG, WU MINGKE, JI YALIANG, WU HAO, JIANG MIN
• Format: Patent
• Language: Chinese; English
• Published: 13.04.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for fermented production of succinic acid on the basis of an electrochemical system for regulating intracellular reducing power regeneration. The method comprises three steps including strain activation, seed culture and anaerobic fermentation production of succinic acid, anaerobic fermentation adopts electrochemical fermentation, an electron carrier is added to a fermentation medium, the corresponding voltage is applied to a cathode, and the concentration of the electron carrier is in a range of 0.01-0.5 mmol/L. the electron carrier is neutral red or riboflavin. The cathode voltage applied to the cathode is scanned and determined in the range from 0.1 V to 1 V with the cyclic voltammetry at the scanning speed of 5-10 mV/s. When fermentation is performed in an electrochemical device under the anaerobic condition, the total amount of intracellular NADH is increased under the assistance of the electron carrier, the intracellular reducing power (NADH/NAD+) level is tripled, the s