Serum-free medium used for culturing virus, and preparation method thereof

The invention relates to a serum-free medium used for culturing virus. The serum-free medium is prepared from, by weight, 80 to 90% of a culture solution, 4 to 10% of amino acids, 3 to 10% of a serum alternative factor, and 0.05 to 0.5% of an antioxidant. The invention also provides a preparation me...

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Bibliographic Details
Main Authors GAO YAN, TANG ZHAOXIN, CHEN RUIAI, ZHANG WENYAN, XU JIAHUA, HE FANG
Format Patent
LanguageChinese
English
Published 30.03.2016
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a serum-free medium used for culturing virus. The serum-free medium is prepared from, by weight, 80 to 90% of a culture solution, 4 to 10% of amino acids, 3 to 10% of a serum alternative factor, and 0.05 to 0.5% of an antioxidant. The invention also provides a preparation method of the serum-free medium. In the preparation method, the culture solution, the amino acids, the serum alternative factor, and the antioxidant are mixed at the above ratio so as to obtain the serum-free medium. The serum-free medium is used for virus culturing, is convenient for purifying of virus liquid after virus breeding, and is capable of reducing vaccine production cost; and the preparation method is simple, and is convenient for operation.
Bibliography:Application Number: CN201510965947