一种液相合成多肽的方法及应用

• Format: Patent
• Language: Chinese
• Published: 30.04.2019
• Subjects: CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE

本发明涉及一种在液相合成多肽的方法及应用。将氨基酸溶解在水或有机溶剂或离子液体中，得到氨基酸溶液；将含有氨基酸的溶液直接装入冷等离子体放电器中，在搅拌条件下，通入等离子体放电气体；利用高压电源在电极两端施加200～5000V的直流或交流电使放电气体放电，形成的等离子体在液体表面促使氨基酸发生缩合反应，每次反应时间为1～10min，反应后补充处理过程挥发的溶剂，重复反应3到15次；反应后的溶液经高效液相色谱分离，可得到多肽产品。本发明减少多肽合成过程中化学试剂的使用，减少反应时间。利用冷等离子诱发缩合反应，在不添加缩合剂的情况下，一步完成了氨基酸合成多肽的过程，高效环保。 The invention relates to a method used for synthesizing polypeptides in a liquid phase, and applications thereof. The method comprises following steps: amino acids are dissolved in water, or an organic solvent, or an ionic liquid so as to obtain an amino acid solution; the amino acid solution is delivered into a cold plasma discharger directly, and a plasma discharge gas is added with stirring; discharging of the plasma discharge gas is carried out via applying 200 to 5000V direct or alternating current onto the two ends of an electrode using a high voltage power supply, so that an obtained plasma is capable of promoting condensation reaction of amino acids on liquid surface, each reaction time is controlled to be 1 to 10min,