Taxus chinensis cell culture medium promoting paclitaxel excretion

• Main Authors: XIA XINCHENG, XIA XUECHENG
• Format: Patent
• Language: Chinese; English
• Published: 16.03.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a taxus chinensis cell culture medium promoting paclitaxel excretion. According to the culture medium, a traditional autotrophic culture medium is adopted for providing a nitrogen source and a phosphorus source, a certain quantity of calcium ions are added, and on this basis, it is unexpectedly found that the yield of paclitaxel can be increased by adding specific types of traditional Chinese medicinal materials with specific ingredients. Based on the helpful finding, roots of wisteflower wisteria, sesamum indicum seeds, tinospora sinensis(Lour.)Merr. common bluebeard herb, gynura japonica, stephania sinica diels, pogonatherum crinitum, saffron crocus, flos imperatae and other traditional Chinese medicinal materials originally used for treating human diseases are preferably selected through an experimental means, an outstanding culture effect of the obtained culture medium is unexpectedly achieved, the paclitaxel yield of taxus chinensis cells is remarkably increased, and meanwhile the