Lipase immobilization carrier, enzyme immobilization method and method for improving resolution performance

• Main Authors: YE PENG, HU PENGCHENG
• Format: Patent
• Language: English
• Published: 24.02.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

The invention relates to a lipase immobilization carrier, a lipase immobilization method and a method for improving chiral resolution performance by using the immobilized lipase; the lipase immobilization carrier is prepared by introducing an amino macromolecular carrier material in a molecular structure and carrying out dehydration condensation with bile acid or bile acid salt. According to the lipase immobilization carrier, through a hydrophobic effect produced by bile acid and lipase, a 'lid' of a lipase active site is induced to be opened and acts with a lipase 'lid' inner side hydrophobic part to induce lipase to be immobilized at the surface of the modified macromolecular carrier material, and thus not only is the stability of the immobilized lipase improved, but also the catalytic activity of the lipase is ensured.