Multi-PCR primer for detecting EGFR/KRAS/BRAF genetic mutation locus based on next-generation sequencing technology and method and application

• Main Authors: GE LIANGJIN, LIU SONG, LIU LICHUN, LIN QUNTING, LI GAILING, DENG LIWEI, ZENG LIDONG
• Format: Patent
• Language: English
• Published: 17.02.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a multi-PCR primer for detecting EGFR/KRAS/BRAF genetic mutation locus based on a next-generation sequencing technology. The multi-PCR primer comprises two or more pairs of 14 pairs of primers shown as SEQ ID NO: 1-SEQ ID NO: 28; preferably, the multi-PCR primer comprises a primer pair including any two or three genes of amplification EGFR, KRAS and BRAF genes at a minimum, wherein each gene amplifies one or more exon regions, and one or more primer pairs are adopted for each exon region. The multi-PCR primer set can be used for efficient detection of diversity of EGFR/KRAS/BRAF genetic mutation locus and can cover 18th-21th EGFR exon, the second KRAS exon and the 15th BRAF exon.