Method for preparing homogenous liquid particle strain by utilizing nicandra seeds

The invention discloses a method for preparing a homogenous liquid particle strains by utilizing nicandra seeds. The method comprises the steps of seed sterilization, slope culture medium preparation, inoculation, liquid medium preparation and shaking cultivation; specifically, packaging the nicandr...

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Bibliographic Details
Main Authors GUO YONGHONG, LUO XIAOKUN, SHANG LUE, LIU SHAOXIONG, LI JIANYING, HUA RONG
Format Patent
LanguageEnglish
Published 17.02.2016
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for preparing a homogenous liquid particle strains by utilizing nicandra seeds. The method comprises the steps of seed sterilization, slope culture medium preparation, inoculation, liquid medium preparation and shaking cultivation; specifically, packaging the nicandra seeds and sterilizing; preparing a PDA slope culture medium and sterilizing; putting the nicandra seeds into the slope culture medium, uniformly paving the nicandra seeds to be as a seed layer; inoculating targeted test tube strains onto the surface of the seed layer, and cultivating the strains at 22-25 DEG C in dark for 6-8 days till targeted hypha coats the surfaces of the nicandra seeds; preparing the liquid medium by per 1000ml from the following ingredients: 180-220 g of potato, 18-22 g of glucose, 0.4-0.6 g of KH2PO4, 0.4-0.6 g of K2HPO4, 0.4-0.6 g of MgSO3 and 3.2-3.8 g of agar powder, wherein a PH value of the liquid medium is 6.5-7.5; inoculating the nicandra seeds coated with the targeted hypha into the liquid medium, and performing shaking cultivation on the nicandra seeds at 22-25 DEG C in dark for 8-12 days, thereby obtaining targeted homogenous liquid particle strains. The strains prepared by the method provided by the invention have the advantages that the distribution of ball strains is uniform, and the homogenous liquid strains can be obtained without external-force crushing and pulping; the damage of external force on the ball strains is reduced, and the integral quality of the ball strains is improved.
Bibliography:Application Number: CN201510827464