Kit for quickly detecting sinotype deletional thalassemia based on hydrolysis probe method

The invention discloses a kit for quickly detecting sinotype deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R a...

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Bibliographic Details
Main Authors PANG WANRONG, SUN LEI, LONG JU
Format Patent
LanguageEnglish
Published 16.12.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a kit for quickly detecting sinotype deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R amplification products, wherein the DBT-F is 5'-GGGTGAGGAACAATTGAA AC-3', the DBT-R is 5'-ACCACATCCCTAACACAAC-3', and the DBT-probe is 5'-ROX-ACTATCA CACCCTGCTTACCTCTGATGGA-BHQ-2-3'. The kit can be used for achieving detection of DBT deletional thalassemia alleles through single-tube one-time reaction and has high sensitivity, stability and accuracy and higher specificity.
Bibliography:Application Number: CN20151701635