Preparation of fructus forsythiae active site and application of fructus forsythiae active site to Parkinson disease

• Main Authors: SONG XIUYUN, JIANG JIANSHUANG, ZHANG PEICHENG, ZHANG SHUAI, FENG ZIMING, CHEN NAIHONG, YANG YA'NAN, ZHANG FAN
• Format: Patent
• Language: English
• Published: 16.12.2015
• Subjects: CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; DERIVATIVES THEREOF; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SUGARS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The invention relates to a preparation method of a common traditional Chinese medicine of a fructus forsythiae active site. The method comprises: pulverizing a medicinal material of fructus forsythia; performing reflux extraction with a solvent, such as water or ethanol; concentrating the extracting liquid at a reduced pressure to obtain extractum; performing extraction through a plurality of organic solvents and recycling the organic solvents of the site extracted by n-butyl alcohol; dispersing and dissolving the site extracted by n-butyl alcohol in a proper amount of distilled water; taking the obtained supernate out and putting the supernate in a macroporous adsorption resin with weak polarity or medium polarity; and performing elution in a water-alcohol system to obtain the fructus forsythiae active site. According to the method, a separation method and a modern spectroscopic analysis method for a plurality of chemical components are utilized to separate and identify chemical components in the active site, thereby obtaining 15 compounds, 9 of which are new compounds. In vitro, the fructus forsythiae active site has an obvious function of preventing neurocyte damage due to rotenone, and in vivo, the fructus forsythiae active site can significantly increase the number of TH positive neuron, due to rotenone, of a substantia nigra zona compact of a rat and improve a Parkinson symptom of the rat. Therefore, the fructus forsythiae active site can be prepared into a medicine for preventing and curing the Parkinson disease.