Universal protein overexpression tag comprising ramp function, and application thereof

• Main Authors: MIN SA-YOUNG, PARK WON-JI, LEE EUN-BIN, YOU SUNG-HWAN, KIM GEUN-JOONG, LEE JIN-YOUNG
• Format: Patent
• Language: English
• Published: 09.12.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a ramp tag capable of solving the instability in translation speed resulting from poor compatibility between codons in an exotic gene and a host when expressing a recombinant protein in E. coli. Unlike the conventional codon optimization or codon deoptimization method for solving the problem of rare codons, the present invention increases the expression efficiency of a target protein by merely having the ramp tag to be fused with a target gene or independently expressed, without changing the original codon sequence, thereby allowing tRNA to be reused. Thus, the present invention relates to a novel method for increasing recombinant protein expression which is capable of reducing costs and time in comparison to the codon optimization method, which artificially synthesizes DNA sequences. Therefore, it is expected that the method of the present invention will be able to be used in the production of high value-added pharmaceuticals or industrial enzymes.