Modified glycine oxidase

• Main Authors: TAKAHASHI KAZUTOSHI, HOSHINO WATARU, KODAMA YUYA, ITO MOEMI
• Format: Patent
• Language: English
• Published: 25.11.2015
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention provides a means and a method that are useful in glycine concentration measurement. Specifically, the present invention provides: a modified enzyme in which at least one amino acid residue is mutated so as to improve the properties of glycine oxidase that relate to glycine measurement (for example, the activity of glycine oxidase with respect to glycine, the thermal stability of glycine oxidase, and the substrate specificity of glycine oxidase with respect to glycine); and a glycine analysis method which includes using the modified enzyme to measure glycine contained in a sample to be examined, and the like.