Method for synthesizing cyclopeptide by multicomponent reaction under enzyme catalysis actions

• Main Authors: ZHANG BO, YANG RUNLIN, YANG MIN, PAN DONGHUI, YAN JUNJIE, ZHAO FUKUAN, XU YUPING, WANG LIZHEN
• Format: Patent
• Language: English
• Published: 25.11.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention discloses a method for synthesizing cyclopeptide by multicomponent reaction under enzyme catalysis actions, which comprises the following step: in a liquid-phase system, reacting diamine, isonitrile and aldehyde under the catalytic action of an enzyme to obtain the cyclopeptide. The diamine is introduced into the multicomponent reaction, and is subjected to intermolecular chain extension and intramolecular cyclization under the catalytic action of the enzyme to prepare the cyclopeptide containing different quantities of peptide bond units. The method can efficiently construct the straight-chain peptide molecule without any other catalyst or condensing agent, and implements the synthesis of the cyclopeptide under the action of in-situ induced cyclization; and especially under the action of water, the purity of the generated cyclopeptide is higher than 95%.