SS-PCR (species-specific polymerase chain reaction) primer and kit for quickly identifying bactrocera correcta

• Main Authors: WU ZHIYI, WANG NIANWU, YU YUN, HUANG KEHUI, HUANG ZHEN, ZHANG WEI, LOU DINGFENG, GUO QIONGXIA
• Format: Patent
• Language: English
• Published: 18.11.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of molecular biology, particularly to an SS-PCR (species-specific polymerase chain reaction) primer and kit for quickly identifying bactrocera correcta. The SS-PCR primer pair for quickly identifying bactrocera correcta comprises the following primers: LJF400: 5'-ACTCTGTTTAGCCGGGATCT-3' and LJR564: 5'-CCGGCTAAAACTGATGGAGA-3'. The method for quickly identifying bactrocera correcta by using SS-PCR established by the invention has the advantages of being quick, easy and convenient, high in specificity and sensitivity, and the like, the identification of bactrocera rubigina can be achieved within 8 hours, and the demand of quick customs clearance of port inspection and quarantine can be met.