Method for inducing umbilical cord mesenchymal stem cells to be differentiated into leydig cells through gold nanoparticle medicated gene transfection

The invention provides a method for inducing umbilical cord mesenchymal stem cells to be differentiated into leydig cells through gold nanoparticle medicated gene transfection. Plasmids with SF-1 (seroidogenic factor 1) genes and LHR (luteotropic hormone receptor) genes are cotransfected into the um...

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Bibliographic Details
Main Authors CHANG MANG, FENG WENFENG, HUANG PENG
Format Patent
LanguageEnglish
Published 18.11.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides a method for inducing umbilical cord mesenchymal stem cells to be differentiated into leydig cells through gold nanoparticle medicated gene transfection. Plasmids with SF-1 (seroidogenic factor 1) genes and LHR (luteotropic hormone receptor) genes are cotransfected into the umbilical cord mesenchymal stem cells through gold nanoparticles, then the umbilical cord mesenchymal stem cells are induced by compounds or growth factors to be directionally differentiated into the leydig cells. The leydig cell differentiation method is efficient and safe, the differentiated leydig cells can serve as a research substitution model and can be used as seed cells of clinical cell treatment of the leydig cells, and an application prospect of leydig cell regeneration replacement treatment is widened.
Bibliography:Application Number: CN20151498088