种棘头梅童鱼精子超低温冷冻保存方法

• Format: Patent
• Language: Chinese
• Published: 05.04.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; TRAPPING

种棘头梅童鱼精子超低温冷冻保存方法，其特征是选择海区捕获性成熟的棘头梅童鱼雄鱼获取精巢，用剪刀将精巢破碎，沾取精液于海水中激活后，立即在光学显微镜下观察精子活力，有活动能力精子数占总精子数的比例在50％以上的精液用于超低温冷冻保存；破碎后的精巢按体积比1:4-1:5与冷冻保护液混匀，去除精巢碎块后将精子混合液分装于2ml冻存管中，每管装入1.5ml；将装有精子混合液的冻存管室在温下平衡2min，液氮面上方10-15cm的液氮蒸汽中平衡15min后放入液氮-196℃中冷冻保存；冷冻精子于37℃水浴中解冻复活，解冻后光学显微镜下检查精子活力。 The invention discloses an ultralow temperature cryopreservation method of collichthys lucidus sperm. The method is characterized by comprising the following steps: selecting sexually matured male collichthys lucidus captured in the sea, obtaining spermary from the male collichthys lucidus, grinding the spermary by scissors, dipping the seminal fluid in sea water to activate the sperm, immediately observing the sperm activity by an optical microscope, wherein the seminal fluid with an active sperm ratio of 50% or more can be applied to cryopreservation; evenly mixing the grinded spermary and a cryoprotective solution according to a volume ratio of 1:4-1:5, removing the spermary fragments, then filling 1.5 mL of mixed sperm solution into 2 mL