DNA label sequence, sequencing library construction method and kit
The invention relates to a DNA label and PCR primer pairs with the 5'-end connected with the DNA label, and provides a sequencing library construction method adopting the DNA label and the PCR primer pairs, and a kit composed of the DNA label and the PCR primer pairs. The DNA label and amplimer...
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Main Authors | , , |
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Format | Patent |
Language | English |
Published |
11.11.2015
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to a DNA label and PCR primer pairs with the 5'-end connected with the DNA label, and provides a sequencing library construction method adopting the DNA label and the PCR primer pairs, and a kit composed of the DNA label and the PCR primer pairs. The DNA label and amplimers form a detection product with optimized and balanced specificity, sensitivity and repeatability, the detection product can be used to detect 1-20 different sources of a sample once, and can accurately distinguish the base sequence of various sample sources. The coincidence rate of high flux sequencing and a sequencing method reaches up to 100%. |
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Bibliography: | Application Number: CN20151383487 |