Method and composition used for obtaining neuron-like cells from non-neuronal cells via reprogramming

• Main Authors: DENG HONGKUI, ZUO XIAOHAN, LI XIANG
• Format: Patent
• Language: English
• Published: 11.11.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a method and a composition used for obtaining neuron-like cells from non-neuronal cells via reprogramming. The composition comprises a neuron-like characteristic chemical inducer; the neuron-like characteristic chemical inducer comprises an arbitrary combination of the following ingredients: 1) a cyclic adenosine monophosphate agonist; 2) neurogenic small molecules; 3) glycogen synthase kinase inhibitors, 4) TGF beta receptor inhibitors; 5) BET family bromodomain inhibitors; and 6) a selective inhibitor of Rho-associated, coiled-coil containing protein kinase and/or a p38 mitogen-activated protein kinase inhibitor; wherein 6) is a selective ingredient. The composition used for providing non-neuronal eukaryotic cells with neuron-like characteristics is capable of producing more than 90% TUJ1 positive cells in 16 days of induction. The neuron-like cells obtained via chemical induction possess neuronal specific expression profiles, are capable of generating action potential, and forming functional synapses via further maturation promotion.