Breeding method for hypsizigus marmoreus strains

• Main Authors: LIU QUNTAO, ZHOU YUANKE, CHEN YONGMIN, LIU XIA
• Format: Patent
• Language: English
• Published: 04.11.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a breeding method for hypsizigus marmoreus strains. The breeding method comprises steps as follows: (1), a culture medium is prepared; (2), bottling and sterilizing are performed; (3), the culture medium is poured into culture vessels; (4), inoculation is performed: a black line is marked on a bottom cover of each culture vessel to divide the surface of the bottom cover into two parts equally, and hypsizigus marmoreus mother strains and trichoderma viride pathogenic bacteria are inoculated on two opposite points 3 cm away from the center of a flat plate respectively; (5), culturing is performed: the culture vessels are put in a dark and airtight environment at the temperature of 22 DEG C after inoculation for culturing, and the diameter of a bacterial colony in each culture vessel is recorded day by day; (6), after culturing, the antibacterial rate of the hypsizigus marmoreus strains to the trichoderma viride pathogenic bacteria in each culture vessel is calculated, and the hypsizigus marmoreus strains with a higher antibacterial rate in one culture vessel are selected as target strains. The hypsizigus marmoreus strains with higher trichoderma viride activity resistance can be cultured without mutagenesis with physical and chemical methods, the method is simple, convenient and easy to popularize, energy is seldom consumed, no pollution is caused to the environment, and the breeding method accords with the industrial policy about environment protection in China.