狂犬病病毒的纯化方法

• Format: Patent
• Language: Chinese
• Published: 06.11.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

本发明的目的是种纯化狂犬病病毒的方法，它包括唯的离子交换色谱法步骤，所述步骤是阳离子交换色谱法步骤，其中：A、让被病毒感染的细胞的培养物的上清液与阳离子交换色谱担体接触，该担体含有接枝磺基异丁基基团的聚甲基丙烯酸酯基体，以便让狂犬病病毒结合在担体上，以及，B、从其担体上洗脱该病毒。 The subject of the invention is a method for purifying the rabies virus, comprising a single ion-exchange chromatography step, said step being cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography support comprising a polymethacrylate matrix onto which sulfoisobutyl groups have been grafted such that the rabies virus binds to this support, and; b) the virus is eluted from its support.