Diagnostic method for rapidly detecting bacterium metal beta-lactamase drug resistance gene VIM

• Main Authors: HUANG XI, ZHANG XUEFEI, TIAN GUOBAO, ZHONG LANLAN
• Format: Patent
• Language: English
• Published: 07.10.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an LAMP kit and method used for rapidly detecting bacterium metal beta-lactamase drug resistance gene VIM. The kit comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. The method is implemented as SEQ ID NO.1-6 and includes the steps that DNA of a to-be-detected bacterium genome is extracted, six specific primers and a kind of DNA polymerase with the strand displacement activity are adopted, the DNA sample is amplified at 60 DEG C to 65 DEG C, and a user adds color developing agents to observe changes in the color in a reaction tube to judge whether amplifying is achieved or not. The LAMP kit and method have the advantages of being rapid, efficient, easy and convenient to operate, high in specificity, high in sensitivity, easy and convenient to detect, suitable for site detection and the like, and is suitable for application and popularization.