Method for enhancing glucose conversion rate of Aspergillus niger saccharifying enzyme

The invention discloses a method for enhancing glucose conversion rate of an Aspergillus niger saccharifying enzyme. The composite of an agdB-gene-inactivated Aspergillus-niger-secreted saccharifying enzyme and a debranching enzyme is utilized to convert starch sugar. The alpha glucosaccharase agdB...

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Bibliographic Details
Main Authors BAI AIXI, BIAN FURONG, WANG CAIMEI, LI FENG, ZHANG YIN, SUN YAN
Format Patent
LanguageEnglish
Published 07.10.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for enhancing glucose conversion rate of an Aspergillus niger saccharifying enzyme. The composite of an agdB-gene-inactivated Aspergillus-niger-secreted saccharifying enzyme and a debranching enzyme is utilized to convert starch sugar. The alpha glucosaccharase agdB gene is inactivated to lower the saccharifying enzyme fermentation activity of Aspergillus niger, and the saccharifying enzyme can be directly compounded with the debranching enzyme to convert glucose, thereby obviously enhancing the DX value.
Bibliography:Application Number: CN20151430731