Novel glycosaminoglycan analogue and synthetic method thereof, and application of novel glycosaminoglycan analogue in invitro embryonic stem cell proliferation and directional neural differentiation

• Main Authors: CHEN GAOJIAN, CHEN HONG, DING KAIGUO, WANG MENGMENG, YUAN LIN, LYU ZHONGLIN
• Format: Patent
• Language: English
• Published: 23.09.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The invention provides a novel glycosaminoglycan analogue and a synthetic method thereof, and an application method of the novel glycosaminoglycan analogue in invitro embryonic stem cell proliferation and directional neural differentiation. The synthetic method is characterized by comprising the following steps: adding a sodium p-styrenesulfonate monomer or/and a 2-methacrylamide glucopyranose monomer into a mixed solvent according to a certain proportion, wherein the mixed solvent is a mixture of water and N,N-dimethylformamide; and carrying out polymerization by applying a method of reversible addition-fragmentation chain transfer(RAFT) so as to respectively obtain a plurality of polymers of the sodium p-styrenesulfonate monomer or/and the 2-methacrylamide glucopyranose monomer in different proportions. According to the invention, a macromolecule substance capable of promoting an embryonic stem cell to proliferation in invitro and inducing the embryonic stem cell to realizing highly-efficient directional neural differentiation is obtained with a RAFT synthetic technology by utilizing a sulfonate-group-contained monomer and a sugar-contained monomer and controlling relative proportion of the sulfonate-group-contained monomer and the sugar-contained monomer to adjust the structure of a polymer.