F3-generation rapid-growth flounder pure line construction method

• Main Authors: QI WENSHAN, LI HONG, CHEN HONGLIN, SONG LINI, CHEN SONGLIN, LI XIANGKONG, TIAN YONGSHENG
• Format: Patent
• Language: English
• Published: 23.09.2015
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; FORESTRY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HUNTING; NEW BREEDS OF ANIMALS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; TRAPPING

A breeding technique method for selecting, inducing and cultivating excellent flounder lines belongs to the technical field of marine organisms. The method mainly comprises screening and construction of F1-generation and F2-generation rapid-growth flounder excellent families, induced establishment of mitotic and meiotic F3-generation gynogenetic pure lines, and genetic monitoring of flounder pure lines. According to the method, a rapid-growth F1-generation family F0750 and a rapid-growth F2-generation excellent family F09119 are screened and constructed; a mitotic F3-generation gynogenetic line F1346 and a meiotic F3-generation gynogenetic line F1324 are constructed through heterogenous frozen semen induction, hydrostatic pressure induction and cold shock induction and are compared on growth with an inbred line F1313, wherein the growth speed of the F1346 is obviously higher than those of the F1324 and the F1313; the genetic monitoring is performed through microsatellite markers scaffold288_1492; the genetypes of the F1346 and the F1324 are pure AA and BB respectively, while that of the F1313 is hybrid. Therefore, excellent pure lines of rapid-growth new flounder species can be constructed.