Cordate houttuynia wall-breaking decoction pieces fingerprinting construction and quality detection method thereof

• Main Authors: PENG LIHUA, CHENG JINLE, XU JIYIN, WANG HUILING
• Format: Patent
• Language: English
• Published: 19.08.2015
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a cordate houttuynia wall-breaking decoction pieces fingerprinting construction and a quality detection method thereof. The method takes quercitrin as a contrast, when column temperature is 30 DEG C, wavelength is 326nm, acetonitrile is a mobile phase A, 0.1% of phosphoric acid solution is a mobile phase B, gradient elution is 0-45 minutes, the mobile phase A is changed from 10%-27%, the samples with more than 10 batches are performed with an HPLC analysis, and a common mode standard atlas and determine index can be established. The quality of the samples to be detected can be detected by comparing the atlas of the sample to be detected under same chromatogram condition. The invention firstly aims at the cordate houttuynia wall-breaking decoction pieces to establish the HPLC common mode standard atlas and quality detection method. The atlas comprehensively covers the atlas information of the main active component of the cordate houttuynia wall-breaking decoction pieces, has the advantages of strong specialization, and rapid and accurate detection; and can effectively control the integral quality of medicinal material, wall breaking powder and the wall breaking decoction pieces.