Method for expressing proteins by using plant petal cell protoplast

• Main Authors: BAO FEI, ZHANG QIXIANG, WANG JIA, CHENG TANGREN, SUN LIDAN, YANG WEIRU, ZHOU YUZHEN, MA KAIFENG, PAN HUITANG
• Format: Patent
• Language: English
• Published: 12.08.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for expressing proteins by using plant petal cell protoplast. The method comprises: (1) adopting plant petals as a material, and carrying out enzymolysis to obtain petal cell protoplast; (2) standing the petal cell protoplast for 20-40 min at a temperature o...