Method for expressing proteins by using plant petal cell protoplast

The present invention relates to a method for expressing proteins by using plant petal cell protoplast. The method comprises: (1) adopting plant petals as a material, and carrying out enzymolysis to obtain petal cell protoplast; (2) standing the petal cell protoplast for 20-40 min at a temperature o...

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Main Authors BAO FEI, ZHANG QIXIANG, WANG JIA, CHENG TANGREN, SUN LIDAN, YANG WEIRU, ZHOU YUZHEN, MA KAIFENG, PAN HUITANG
Format Patent
LanguageEnglish
Published 12.08.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to a method for expressing proteins by using plant petal cell protoplast. The method comprises: (1) adopting plant petals as a material, and carrying out enzymolysis to obtain petal cell protoplast; (2) standing the petal cell protoplast for 20-40 min at a temperature of 2-8 DEG C; and (3) transforming plasmid into the protoplast by using a PEG and Ca<2+>-mediated method, and expressing the target protein. Compared with the method in the prior art, the method of the present invention has the following beneficial effects that: the target protein can be expressed in a short time, the protoplast protein expression system can be used for research on target protein subcellular positioning and protein molecule interaction, the activity of the protoplast is normal, and the target protein expression detection results show that the method can be used for research on gene transcription level, protein expression level and substance metabolism level change in living cells and is the rapid and convenient target gene function research method.
Bibliography:Application Number: CN20151172958