Method for extracting DNA (deoxyribonucleic acid) by virtue of combination of magnetic bead method and centrifuge shield

The invention discloses a method for extracting DNA (deoxyribonucleic acid) by virtue of the combination of a magnetic bead method and a centrifuge shield. The method comprises the following steps: (1) putting a detection material into an inner sleeve of the centrifuge shield, uniformly mixing a TES...

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Bibliographic Details
Main Author QI JIELI
Format Patent
LanguageEnglish
Published 12.08.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for extracting DNA (deoxyribonucleic acid) by virtue of the combination of a magnetic bead method and a centrifuge shield. The method comprises the following steps: (1) putting a detection material into an inner sleeve of the centrifuge shield, uniformly mixing a TES buffer solution, sodium dodecyl sarcosinate and protease K according to a volume ratio, then adding the mixture into the inner sleeve of the centrifuge shield, and putting the centrifuge shield on a metal bath to perform pyrolysis; (2) adding an adsorption solution and centrifuging again; (3) removing the inner sleeve, adding the adsorption solution, adding a magnetic bead suspension, and putting on an automatic vortex instrument to perform adsorption; (4) putting on a magnetic frame to suck away a supernatant, and then putting into a centrifuge to suck away residual liquid again; (5) adding absolute alcohol for whirling, putting on the magnetic frame to fully suck away the supernatant, and putting into the centrifuge to suck away the residual liquid again by centrifuging; (6) drying magnetic beads, adding an eluent to whirl fully, and then putting on the metal bath for incubation; and (7) performing amplification in a PCR (polymerase chain reaction) amplification solution. The method disclosed by the invention is high in detectable rate, and the final template keeping amount reaches 50-70%, so that the method is particularly suitable for detecting micro detection materials, and the field DNA detection material containing more than 100pg of templates can be detected.
Bibliography:Application Number: CN20151232692