High-efficiency DNA hybridization solution and application thereof

• Main Authors: WANG ZHENGLONG, LI JIGUANG, ZHENG DUNWU, YANG LONGXING, XIE PEI
• Format: Patent
• Language: English
• Published: 29.07.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a high-efficiency DNA hybridization solution which is composed of NaH2PO4, urea and sodium dodecyl sulfate; the content of NaH2PO4 is 0.5-10% (w/v), the content of urea is 10-70% (w/v); the content of sodium dodecyl sulfate is 0.2-30% (w/v); NaH2PO4, urea and sodium dodecyl sulfate are respectively weighed in proportion to be dissolved in quantificational distilled water; all the components in distilled water are added regardless of order. The high-efficiency DNA hybridization solution is simple in composition and low in cost, hybridization temperature can be reduced, and urea in the high-efficiency DNA hybridization solution is odorless, tasteless, safe and environmental-friendly. Experimental results are comparable with those of foreign well-known brands, and the cost of the high-efficiency DNA hybridization solution is only 7.7% of those of the foreign well-known brands.