Method for rapidly propagating through adoption of aristolochia fordiana leaves

• Main Authors: CHEN QIANPING, GU XIAOYU, PAN LIMEI, HUANG QINFEN, BAI LONGHUA
• Format: Patent
• Language: English
• Published: 15.07.2015
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention provides a method for rapidly propagating through adoption of aristolochia fordiana leaves. The method comprises the following steps: preparing an aristolochia fordiana sterile explant; performing callus inducing, namely removing veins of the sterile explant, dicing, inoculating an induction culture medium with the diced sterile explants, performing induction culture to obtain a callus; performing callus differentiating, namely inoculating a differential culture medium with the callus to obtain multiple shoots; hardening seedlings, namely inoculating a seedling culture medium with the multiple shoots, performing the seedling culture to obtain a test-tube plantlet; rooting, namely transplanting the test-tube plantlet into a rooting culture medium to obtain a test-tube plantlet with the root; and hardening the plantlet and transplanting, namely hardening the plantlet to obtain a complete plant, and then transplanting into a matrix to culture. The leaves are used as the explant, the material taking is convenient; a stem is saved so that the whole strain collecting is avoided, an aristolochia fordiana resource is saved, and the demands of the market to the aristolochia fordiana can be satisfied.