Method for preparing recombinant-penicillium amagasakiense glucose oxidase and application of recombinant-penicillium amagasakiense glucose oxidase

• Main Authors: QIN LI'NA, MA ZHIZHI, DONG ZHIYANG, LIN JIE, HUANG ZHENBANG, CHEN XIUZHEN
• Format: Patent
• Language: English
• Published: 17.06.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for preparing recombinant-penicillium amagasakiense glucose oxidase and application of the recombinant-penicillium amagasakiense glucose oxidase. Recombinant trichoderma reesei Tu6 delta tku70::Pgod constructed by introducing a Pgod gene into trichoderma reesei Tu6 delta tku70 can successfully express the recombinant-penicillium amagasakiense glucose oxidase, the expression quantity is high, and the enzyme activity in fermentation broth can achieve 28 U/mL. The recombinant-penicillium amagasakiense glucose oxidase in the fermentation broth is subjected to ni-sepharose purification, the enzyme activity in a protein solution obtained by ultrafiltration concentration can achieve 330 U/mL, and the specific activity of the recombinant-penicillium amagasakiense glucose oxidase is 407 U/mg protein. The recombinant-penicillium amagasakiense glucose oxidase prepared by the method has good heat stability and good acid-alkali tolerance, the fermentation process is simple, transferring is not needed, raw materials are cheap and are easily obtained, and the cost is greatly reduced. The recombinant-penicillium amagasakiense glucose oxidase can be widely applied to the fields of food and medicine.