Kit for preparing DC-CTL and application of kit

The invention discloses a kit for preparing DC-CTL and an application of the kit. The kit for preparing DC-CTL comprises a reagent for separating single karyocyte, a reagent for inducing DC differentiation and maturation, a tumor antigen activity epitope peptide for sensitizing DC and a reagent for...

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Bibliographic Details
Main Authors ZHANG YUN, JI XILUAN, LUO CHAOXIA, JIANG SHU, YANG SHUN
Format Patent
LanguageEnglish
Published 27.05.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a kit for preparing DC-CTL and an application of the kit. The kit for preparing DC-CTL comprises a reagent for separating single karyocyte, a reagent for inducing DC differentiation and maturation, a tumor antigen activity epitope peptide for sensitizing DC and a reagent for promoting CTL amplification and activation, wherein the reagent for promoting CTL amplification and activation is combination of one or more of an anti-CD3 monoclonal antibody, IFN-gamma, IL-1alpha, PHA, IL-2 and IL-7 and an anti-CD28 monoclonal antibody and anti-ICOS monoclonal antibody. CD8+T cells do not need to be separated from the single karyocyte of peripheral blood by virtue of a flow cytometry or an immunomagnetic bead in preparation of the CTL. The CTL prepared by the method disclosed by the invention is high in purity, high in proliferation capacity, large in number and high in killing activity on tumor cells.
Bibliography:Application Number: CN20141446761