Fusion protein Nt4CL3aPcSTS as well as preparation method and application thereof

• Main Authors: ZHANG XUANYI, FENG JING, ZHANG HONG, LIU HUAN, LUO ZAIQI, GUO HUILI, LIU WENBIN, MA LANQING, ZHAO XIAOMENG, MA TENG, MA YADI, YANG YADONG, MA XUAN, YANG MINGFENG
• Format: Patent
• Language: English
• Published: 13.05.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a fusion protein Nt4CL3aPcSTS as well as a preparation method and an application thereof. The fusion protein Nt4CL3aPcSTS provided by the invention is a fusion protein obtained by fusing protein Nt4CL and protein PcSTS; the protein Nt4CL is a protein composed of the 1st-542nd site amino acid residues of an amino acid sequence shown in SEQ ID No. 1 from N terminal; the protein PcSTS is a protein composed of the 546th-933rd site amino acid residues of the amino acid sequence shown in SEQ ID No. 1 from N terminal. The fusion protein Nt4CL3aPcSTS is capable of catalyzing 4-coumarate and malonyl coenzyme A to generate resveratrol with a bioactivity, and is proved to have the activities of a ligase for 4-coumarate and malonyl coenzyme A, and stilbene synthase.