SNP (Single Nucleotide Polymorphism) detection method of rice high-resistant starch content mutant gene sbe3-rs

• Main Authors: FANG JUN, BAI JIANJIANG, PIAO ZHONGZE, YANG RUIFANG
• Format: Patent
• Language: English
• Published: 29.04.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses an SNP (Single Nucleotide Polymorphism) detection method of a rice high-resistant starch content mutant gene sbe3-rs. The SNP detection method disclosed by the invention comprises the following steps of: designing four gene specific primers by adopting a four-primer amplification refractory mutation system PCR (Polymerase Chain Reaction), namely an ARMS-PCR method, according to the fact that the sbe3-rs gene has single nucleotide polymorphism, adding into the same PCR reaction system to amplify different rice DNAs, and rapidly and accurately detecting whether a rice planting resource or a breeding population thereof contains the mutant gene sbe3-rs according to the characteristic patterns of the PCR thereof. The SNP detection method disclosed by the invention has the characteristics of being high in sensitivity and specificity; the selection efficiency of the high-resistant starch mutant gene sbe3-rs can be increased; and the breeding process of high-resistant starch rice varieties can be accelerated.