Method for separating and purifying pinocembrin, chrysin and galangin from Chinese propolis aqueous extract

The invention relates to a method for separating and purifying pinocembrin, chrysin and galangin from a Chinese propolis aqueous extract. The method adopts Chinese propolis as a raw material, and comprises the following steps: (1) preparing a Chinese propolis aqueous extract; (2) precipitating with...

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Bibliographic Details
Main Authors LIU RENMIN, LI AIFENG, SUN AILING, XUAN HONGZHUAN
Format Patent
LanguageEnglish
Published 29.04.2015
Subjects
CHEMISTRY
HETEROCYCLIC COMPOUNDS
METALLURGY
ORGANIC CHEMISTRY
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Summary:The invention relates to a method for separating and purifying pinocembrin, chrysin and galangin from a Chinese propolis aqueous extract. The method adopts Chinese propolis as a raw material, and comprises the following steps: (1) preparing a Chinese propolis aqueous extract; (2) precipitating with alcohol; (3) coarsely separating with macroporous adsorption resin; and (4) separating and purifying by semi-preparative high performance liquid chromatography. According to the method, the semi-preparative high performance liquid chromatography is used for separating and purifying, a mobile phase is methanol-water, and three kinds of high-purity ingredients, which are identified to be pinocembrin, chrysin and galangin respectively, are obtained. The method is green and environment-friendly in process, does not have serious damage to the environment, and has low comprehensive cost.
Bibliography:Application Number: CN201510029995