种秸秆微氧发酵产氢的方法及装置

• Format: Patent
• Language: Chinese
• Published: 12.10.2018
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

本发明公开了种秸秆微氧发酵产氢的方法，属于可再生能源领域。本发明所述的方法包括以下步骤：(1)采用酸或碱对秸秆进行预处理；(2)产氢混合菌驯化：取厌氧发酵的沼液进行热处理，室温冷却后按10％～50％的接种量接种到培养基中，35℃～55℃温度下，驯化至产氢稳定；(3)秸秆微氧产氢：将秸秆、产氢混合菌混合均匀，调节总固体质量含量为1％～4％，pH值为4.5～9.0，驯化后的产氢混合菌占待发酵物料总体积的20％～40％；将物料加入反应器中进行发酵，控制发酵温度为35℃～55℃，通入氧气，控制氧化还原电位为-300～-150mV，通氧量为70～350ml/kgTS·24h，产氢发酵48～96h。本发明还提供种适用于上述秸秆微氧发酵产氢方法的装置。采用本发明所述的方法和装置可有效提高秸秆发酵产氢效率。 The invention discloses a method for generating hydrogen by virtue of micro oxygen fermentation of straws and belongs to the field of renewable energy sources. The method disclosed by the invention comprises the following steps: (1) pretreating the straws by adopting acid or alkali; (2) domesticating hydrogen-generating mixed bacteria, namely performing heat treatment on biogas slurry which is subjected to anaerobic fermentation, cooling at room temperature, then inoculating to a culture medium according to an inoculum concentration of 10-50%, and domesticating until hydrogen is stably generated at 35-55 DEG C; (3) performing straw micro oxygen hydrogen generation,