Cancer promotion activity quantitative determination method and screening method of cancer promoter

• Main Authors: DENG FURONG, WEI HONGYING, GUO XINBIAO, YANG DI
• Format: Patent
• Language: English
• Published: 15.04.2015
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

A quantitative method for detection of cancer promotion activity of analytes. The method comprises the following steps: selecting a suitable cell model, and dividing the cells into donor cells and recipient cells according to the demand; adding two fluorescent probes in the donor cells and incubating; conducting mixed culture on the donor cells and recipient cells according to the proportion; adding the analyte into a test group of mixed culture cells, wherein the control group is not supplemented; incubating for a certain time, and measuring the number of one fluorescent probe, in the donor cells of test group and control group, transferring to the single positive cells in recipient cells by using a flow cytometry; calculating Te (Transfer effects) values of the two groups, and respectively representing with Tecon and Tetest, wherein Te equals to the number of single positive cells / number of single donor cell * 100%; using TeR to represent the relative value of Te of the analyte, wherein TeR equals Tetest / Tecon * 100%; and evaluating the tumor promotion activity of the analyte according to TeR of the analyte, wherein lower TeR indicates higher activity of the analyte. The invention also relates to application of the above method to in vitro screening of cancer promoters; and when Te is less than 90%, the analyte can be identified as a cancer promoter.