Cell sorting system based on endonuclease specific recognition

• Main Authors: ZHENG DUNWU, LAN TIAN, J.GATES
• Format: Patent
• Language: English
• Published: 08.04.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention relates to a cell sorting system based on endonuclease specific recognition. The cell sorting system based on the endonuclease specific recognition comprises the following components: a first single-stranded nucleotide, a second single-stranded nucleotide, an antibody, a cell sorting medium and a restriction endonuclease, wherein the first single-stranded nucleotide and the second single-stranded nucleotide are complementary, and a complementary region contains a recognition site of the restriction endonuclease; the first single-stranded nucleotide, the second single-stranded nucleotide, the antibody and the cell sorting medium are all modified, the modified first single-stranded nucleotide can be connected with the modified antibody, and the modified second single-stranded nucleotide can be connected with the modified cell sorting medium. The invention also provides a cell sorting method applying the cell sorting system based on the endonuclease specific recognition, and a kit and method for sorting DC (dendritic) cells, NK (natural killer) cells and CIK (cytokine-induced killer) cells from cell samples. By adopting the cell sorting technology provided by the invention, multiple cells can be sorted at the same time, sorting of a large number of delicate cells can be carried out, and the cell yield and survival rate are high.