Method for crushing oil-producing microbial cells of pressurized heating jet flow

• Main Authors: WANG LINGRU, ZHANG ZHE, LIU XIAOJU, LIU YUE, QIAO JIANYUAN, JIN HONGBO, ZHENG BIN, DU FENGGUANG, ZHENG SHIWEN, BI SHENGLEI, XUE BAO, DING LINGFEI
• Format: Patent
• Language: English
• Published: 25.03.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a method for crushing oil-producing microbial cells of pressurized heating jet flow, and belongs to the technical field of cell crushing. The method for crushing oil-producing microbial cells of pressurized heating jet flow comprises the following steps: pressurizing oil-producing microbial cells, and spraying out from a pipeline at a 6-10fold sound velocity; and simultaneously heating the pipeline to 100-200 DEG C, then enabling the sprayed oil-producing microbial cells to hit against inner side walls of two parallel and opposite hard flat plates at angles of 15-165 degrees, and repeatedly hitting for 3-10 times. According to the method provided by the invention, oil-producing microbial cells can be efficiently crushed once; the operation process is simple; the raw materials do not need to be pretreated; a chemical or biochemical reagent does not need to be added; the overall crushing process is friendly to environment, and free of pollution; the crushing rate is 100%; and the method can be widely applied to the technical field of crushing of oil-producing microbial cells.