Betula luminifera tissue culture seedling rooting substrate and rooting and transplanting method thereof

• Main Authors: HUANG HUAHONG, NIU MINGYUE, TONG ZAIKANG, DONG RENHUI, LIN ERPEI, LOU XIONGZHEN
• Format: Patent
• Language: English
• Published: 25.02.2015
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a betula luminifera tissue culture seedling rooting substrate and a rooting and transplanting method thereof and belongs to the technical field of vegetative propagation. The betula luminifera tissue culture seedling rooting substrate is characterized in that the substrate is a mixture of peat, perlite and vermiculite, and a liquid culture medium of MS+cane sugar is adopted to soak the substrate. The rooting and transplanting method mainly includes the steps of selection and preparation of the substrate, inoculation, culturing, seedling hardening and transplanting and the like. The betula luminifera tissue culture seedling rooting substrate and the rooting and transplanting method have the advantages that the substrate is selected to replace a conventional agar solid culture medium, normal growth of betula luminifera tissue culture seedlings is unaffected, and seedling hardening and transplanting survival rate reaches 95%; the rooting and transplanting method is simple and convenient in operation, and the culture medium and the substrate which are adopted are low in cost and easy to obtain, so that production cost is lowered; the betula luminifera tissue culture seedlings obtained through aseptic tissue culture are stable in genetic character, and a basis is provided for genetic improvement of betula luminifera.