Method for promoting rapid propagation of cluster buds of butterfly orchid by utilizing LED (Light Emitting Diode) light source

• Main Authors: REN GUIPING, ZHU GENFA, LIU HAILIN
• Format: Patent
• Language: English
• Published: 18.02.2015
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention belongs to the field of plant tissue culturing, and in particular relates to a method for promoting rapid propagation of cluster buds of a butterfly orchid by utilizing an LED (Light Emitting Diode) light source. The method comprises the following steps: (1) inducing the generation of cluster buds by taking a pedicel of the butterfly orchid as an explant; (2) cutting the cluster buds obtained in the step (1) into simple buds or bunch buds and inoculating the simple buds or the bunch buds into an enrichment medium; and (3) by taking an LED capable of emitting red light or emitting warm white light as a light source, culturing the cluster buds which are obtained in the step (2) and are inoculated into the enrichment medium under the LED capable of emitting red light or emitting warm white light. According to the method, the number of the single buds of the cluster buds is obviously increased; the dry weight, the fresh weight and the plant height of the cluster buds are all increased; and the production cost is lowered and energy conservation is realized.