Preparation method of antioxidant walnut polypeptide health product

• Main Authors: ZHAO LIANGQI, LI PENG, HAO CHANGYAN, DU MEILI
• Format: Patent
• Language: English
• Published: 21.01.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

The invention discloses a preparation method of an antioxidant walnut polypeptide health product. The preparation method comprises the following steps: pre-treating walnut pulp; homogenizing the walnut pulp; extracting walnut proteins; carrying out pre-treatment for enzymolysis; carrying out enzymolysis; separating; desalting; and concentrating and drying to obtain a walnut polypeptide product. Four enzymes: alkaline protease, papain, neutral protease and bromelain are sequentially added in different times in the enzymolysis link. The strength, function and synergy and the like of the effects of the four enzymes which are used for decomposing proteins into polypeptides and amino acids are comprehensively considered and the four enzymes are used for enzymolysis under the optimal enzymolysis conditions, respectively. The reducing capacity of the prepared walnut polypeptide manifests that the absorbancy floats up and down at 0.4, the hydroxyl free radical scavenging rate can reach 95%, the superoxide anion free radical scavenging rate can reach 39.5%, the protein extraction ratio can reach 98.71% and the degree of hydrolysis of the walnut polypeptide can reach 26.37%. Compared with the prior art, those items are greatly enhanced and improved relatively.