Tissue culture and rapid propagation method of E. urophyllaE. Grandis DH32-43

The invention discloses a tissue culture and rapid propagation method of E. urophylla*E. Grandis DH32-43. The method is implemented through the steps of explant establishment, explant disinfection, primary culture, subculture, rooting culture, seedling-hardening, transplantation and the like. Accord...

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Main Authors SHEN YUN, MO JIYOU, WU MANFEN, DENG DONGLI, WU BING, TANG ZAISHENG, WANG JIANZHONG, ZHANG LEI, SHI QIAN, LIANG XIULI, FU MIAO, CHEN YUNCHUN, PANG ZHENWU, WEI BINGJIAN, LAN JUN, SU YONG, CHEN DONGLIN, LI LIFANG, CHEN DEWEN, LIANG TINGHUI, LYU HUALI, HUANG QUANDONG, FENG RONGDI, XIONG TAO
Format Patent
LanguageEnglish
Published 21.01.2015
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Summary:The invention discloses a tissue culture and rapid propagation method of E. urophylla*E. Grandis DH32-43. The method is implemented through the steps of explant establishment, explant disinfection, primary culture, subculture, rooting culture, seedling-hardening, transplantation and the like. According to the invention, special culture medium components are selected for the primary culture, subculture and rooting culture, the E. urophylla*E. Grandis is fast in growth and high in survival rate after being transplanted by virtue of a reasonable seedling-hardening measure, and the purposes of high propagation coefficient and excellent propagation effect are achieved; besides, the method is low in production cost, and the genetic characteristics of obtained tissue culture seedlings are consistent, therefore, the method is an effective way for tissue culture and rapid propagation of eucalyptuses, and has wide application range.
Bibliography:Application Number: CN201410590375